Plant regeneration from protoplasts of Capsicum annuum L. cv. California Wonder

摘要

Plant regeneration from mesophyll protoplasts of pepper, Capsicum annuum L. cv. California Wonder has been demonstrated via shoot organogenesis. Protoplasts isolated from fully expanded leaves of 3-week-old axenic shoots when cultured in TM medium supplemented with 1 mg l~-1 NAA, 1 mg l~-1 2,4-D, 0.5mg l~-1 BAP (CM 1) resulted in divisions with a fre- quency ranging from 20-25/100. Antioxidant ascorbic acid and polyvinylpyrrolidone (PVP) in the medium and incubation in the dark helped overcome browning of protoplasts. Microcalli and macrocalli were formed in TM medium containing 2 mg l~-1 NAA and 0.5 mg l~-1 BAP (CM II) and MS gelled medium containing 2 mg l~-1 NAA and 0.5 mg l~-1BAP (CM III), respectively.