A Comparative Study of Sensitivity of Acetylcholinesterase in Detection of Organophosphorus Pesticide Residues

摘要

In this paper, four enzymes from different sources used for pesticide detection were testednand compared: homogenate from chicken liver, chicken brain and cyprinoids brain were made respectivelynin the laboratory and Acetylcholinesterase (AChE) was purchased from Musca domestica.nThe inhibition of AChE activity under different concentrations of omethoate, trichlorfon andndichlorvos were compared. The results showed that the AChE activity in chicken liver and cyprinoidsnbrain were very low. The capacities of activity inhibition of other two kinds of AChE werenbetter. Chicken brain-esterase’s inhibition rate achieved 24.16%, 50.56%, and 54.1% for 100ug/Lnof omethoate, trichlorfon and dichlorvos respectively. Chicken brain-esterase has high specificnactivity, is low cost, and easy to prepare, and the inhibition of the sensitivity to three organophosphorusnpesticides is significantly higher than enzymes from chicken liver and cyprinoids brain. Sonthe chicken brain-esterase could be a good choice to use to detect pesticide residues in agriculturalnproducts.