A comparative study of sensitivity of acetylcholinesterase in detection of organophosphorus pesticide residues.

摘要

In this paper, four enzymes from different sources used for pesticide detection were tested and compared: homogenate from chicken liver, chicken brain and cyprinoids brain were made respectively in the laboratory and Acetylcholinesterase (AChE) was purchased from Musca domestica. The inhibition of AChE activity under different concentrations of omethoate, trichlorfon and dichlorvos were compared. The results showed that the AChE activity in chicken liver and cyprinoids brain were very low. The capacities of activity inhibition of other two kinds of AChE were better. Chicken brain-esterase's inhibition rate achieved 24.16%, 50.56%, and 54.1% for 100 ug/L of omethoate, trichlorfon and dichlorvos respectively. Chicken brain-esterase has high specific activity, is low cost, and easy to prepare, and the inhibition of the sensitivity to three organophosphorus pesticides is significantly higher than enzymes from chicken liver and cyprinoids brain. So the chicken brain-esterase could be a good choice to use to detect pesticide residues in agricultural products.