Cytotoxic Effect of Photoluminescent RE~(3+) Doped Ca_3(PO_4)_2 Nanorods on Breast Cancer Cell Lines

摘要

Background: Breast cancer reported in the young women exhibits high local and distant recurrence and a poor prognosis. Rare earth doped calcium phosphate phosphors have been extensively investigated due to their unique applications in biomedicine.Methods: In the current study, Tb3+, Ce3+ doped Ca-3(PO4)(2) phosphor were prepared by hydrothermal method at 150 degrees C using citric acid as additive and characterized by PXRD, FT-IR, TG-DTA, EDX, TEM and PL techniques. The photoluminescence properties of Tb3+, Ce3+ doped Ca-3(PO4)(2) phosphor was investigated upon photo excitation at 240 nm. Antiproliferative activity was evaluated by MTT, BrdU proliferation, ELISA, Methylene blue and caspase-3 assays.Results: Ca-3(PO4)(2):Tb3+, Ce3+ phosphor exhibited needle like morphology with length and width similar to 100-500 nm and similar to 40-50 nm, respectively. It exhibited green emission at 550 nm corresponding to D-5(4) -> F-7(5) transition with the CIE coordinates (x, y) as (0.284, 0.614). It also showed remarkable concentration dependent cytotoxicity against MCF-7 as well as MDA-MB 231 cells with negligible cytotoxicity compared to MCF-12A, a human epithelial healthy cell line. It reduced the proliferative index of both cell lines in a concentration dependent manner by inhibiting DNA synthesis and Ki67 protein. It also induced distinct apoptotic changes in the morphology of cell and nucleus and also activated the caspase-3 activity in breast cancer cell lines.Conclusion: The results suggest that Ca-3(PO4)(2):Tb3+, Ce3+ phosphor may be useful for therapeutic application in clinical settings. (C) 2019 AGBM. Published by Elsevier Masson SAS. All rights reserved.