A method of inducing antitumor cytotoxic immunity by dendritic cells transfected with RNA TUMOR CELLS AGAINST BREAST CANCER CELLS

摘要

A method of inducing an antitumor cytotoxic immune response using dendritic cells transfected with RNA of tumor cells against breast cancer cells (breast cancer), comprising co-culturing autologous mononuclear cells (MNC) isolated from peripheral blood dendritic cells (DC) derived from the monocytes adhering MNC fraction of tumor cells transfected with RNA and then exposed to TNF-α stimulated maturation, characterized in that the non-stick cocultivation RAC IOCs and DCs transfected with RNA of autologous tumor cells is performed in the presence of recombinant human interleukin -12 and - recombinant human interleukin -18, and to obtain mature DC after two days of culturing adherent fraction MNC patients with breast cancer received immature DCs transfected with tumor RNA after day stimulated subjected to maturation for a further day.