ANTIMICROBIAL ACTIVITY OF Verbascum speciosum AGAINST THREE BACTERIA STRAINS

摘要

Many plant species among the flora of the world play an important role in traditional medicine. In this respect, our study concerned on the antibacterial activity of aqueous and alcohol extracts obtained from flowers of Verbascum speciosum Schard. (Scrophulariaceae) which were investigated considering its in-vitro antibacterial effect against three bacteria strains namely, Bacillus subtilis, Bacillus cereus, and Escherichia coli. These microorganisms were treated using the disc diffusion method and botanical extracts were applied at three concentrations (12.5, 25 and 50 (ig/ml). The results indicated that these natural materials had an inhibitory effect on the growth of above-mentioned at all used doses. In addition, in both cases, the diameter of inhibition zones arises as the extract concentration increases. On the other hand, results show that etha-nol extract was more effective than aqueous against three microorganisms in all emphasized doses. In case of both aqueous and ethanol extracts, the maximum antibacterial activity was shown against B. cereus followed by B. subtilis, and E. coli was most resistant strain. Our findings indicated that this plant possessed compounds with antimicrobial properties against some microorganisms and might be utilized for developing new drugs. Therefore, these can be under consideration for further investigation on determining their therapeutic potential. Such screening of various natural organic compounds and identifying active agents is needed and beneficial, because successful prediction of drug like properties at the onset of drug discovery will pay off later in drug development.