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首页> 外文期刊>Food science and biotechnology >Rapid Detection of Vibrio parahaemolyticus Strains and Virulent Factors by Loop-mediated Isothermal Amplification Assays
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Rapid Detection of Vibrio parahaemolyticus Strains and Virulent Factors by Loop-mediated Isothermal Amplification Assays

机译：环介导的等温扩增法快速检测副溶血性弧菌菌株和毒性因子

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摘要

A loop-mediated isothermal amplification (LAMP) method for rapid detection of the foodborne Vibrio parahaemolyticus strains and related virulent factors had been developed and evaluated in this study. Six primers, including outer primers, inner primers, and loop primers, were specially designed for recognizing 8 distinct sequences on 3 target genes, which were tlh, tdh, and trh. The detection limits were found to be 100,100 fg, and 1 pg DNA/tube for tlh, tdh, and trh, respectively. Application of LAMP assays were performed on 368 foodborne V. parahaemolyticus strains, the sensitivities of LAMP assays for the tlh, tdh, and trh were 100, 95.6, and 96.4%, and the negative predictive values (NPV) were 100, 84.7, and 93.1%, respectively; with a 100% specificity and positive predictive value (PPV) for all 3 target genes.
机译：环介导的等温扩增（LAMP）方法可以快速检测食源性副溶血性弧菌菌株和相关的强毒因子，并已在本研究中进行了评估。专门设计了六种引物，包括外部引物，内部引物和环引物，以识别3个靶基因上的8个不同序列，分别是tlh，tdh和trh。 tlh，tdh和trh的检出限分别为100,100 fg和1 pg DNA /管。在368例食源性溶血性副溶血性弧菌菌株上进行了LAMP测定，LAMP测定对tlh，tdh和trh的敏感性分别为100％，95.6和96.4％，阴性预测值（NPV）为100、84.7和分别为93.1％；对所有3个靶基因具有100％的特异性和阳性预测值（PPV）。

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  • 来源
    《Food science and biotechnology》 |2010年第5期|p.1191-1197|共7页
  • 作者

    Xihong Zhao; Li Wang; Jin Chu; Yanyan Li; Yanmei Li; Zhenbo Xu; Lin Li; Mark E. Shirtliff; Xiaowei He; Yao Liu; Jihua Wang; Liansheng Yang;

  • 作者单位

    College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China,Key Laboratory for Green Chemical Process of Ministry of Education,School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430073, Hubei, China;

    rnFood Safety Key Laboratory of Guangdong Province, College of Food Science, South China Agricultural University, Guangzhou 510640, China;

    rnCollege of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China;

    rnState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China;

    rnGuangzhou Medical College, Guangzhou 510640, China;

    rnCollege of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China,Department of Microbial Pathogenesis, Dental School, University of Maryland, Baltimore, MD 21201, USA;

    rnCollege of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China;

    rnDepartment of Microbial Pathogenesis, Dental School, University of Maryland, Baltimore, MD 21201, USA,Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, MD 21201, USA;

    rnCollege of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China;

    rnZhongshan Supervision Testing Institute of Quality & Metrology, Zhongshan 528403, China;

    rnGuangzhou Wondfo Biotechnology Company, Guangzhou, China;

    rnCollege of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    loop-mediated isothermal amplification (LAMP); vibrio parahaemolyticus; rapid detection;

    机译：环介导的等温扩增（LAMP）;副溶血性弧菌快速检测;

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