Detection of Recombinant DNA from Genetically Modified Papaya

摘要

A method using polymerase chain reaction (PCR) was developed to detect the genetically modified (GM) papaya (55-1 line), of which the mandatory safety assessment has not been finished in Japan because of insufficient data. The papaya intrinsic papain gene was used as an internal control. The results of PCR amplification of the papain gene segment indicated that a commercial silica membrane type kit (QIAGEN Dneasy plant mini) was useful for extraction of DNA from papaya fruit, but not for extraction from canned papaya fruit.