Purification of hydroperoxide lyase from pea seeds

摘要

Hydroperoxide lyase (HPL) development in pea seeds/seedlings was studied during germination. HPL activity in seedlings appeared after 2 days, increased to a maximum at 4 days (3.7 umol min~(-1) ml~(-1)) and decreased slowly after 8 days (2.56 umol min~(-1) ml~(-1). Protein content decreased over 8 days from a maximum of 12.3 to 2.39 mg/ml. This resulted in an approximate 100-fold increase in specific activity of HPL from 0.01 umol min~(-1) mg~(-1) (day 2) to 1.07 umol min~(-1) mg~(-1) (day 8).