Isolation and characterization of collagen from rhizostomous Jellyfish (Rhopilema asamushi)

摘要

As a part of the study into the potential development of unused and under-used resources, collagen was isolated from the mesogloea of the rhizostomous jellyfish, Rhopilema asamushi, by limited pepsin digestion and characterized. The yield of this col- lagen was high (35.2/100 on a dry weight basis). The primary structure was very similar to that of pepsin-solubilized collagen from edible jellyfish mesogloea, but it was different from those of the co1lagen from edible jellyfish exumbrella and the acid-soluble collagen from its mesogloea. The denaturation temperature (T_d) of 28.8 deg C. This collagen contained a large amount of a fourth subunit that was provisionally designated 74. This collagen may have the chain composition of an αlα2α3α4 heterotetramer.