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Method for producing a functional protein concentrate from giant squid (Dosidicus gigas) muscle

机译:从巨型鱿鱼(Dosidicus gigas)肌肉生产功能蛋白浓缩物的方法

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摘要

The conventional way of processing a protein concentrate (surimi) (and further gelation) from squid muscle poses a number of difficulties, essentially because of the peculiarities of the myofibrillar proteins, which cause extensive very high autolysis and, sometimes, off-flavours and bad taste in the muscle. To overcome these problems, a new procedure has been devised for processing this functional protein concentrate from giant squid (Dosidicus gigas) muscle. It is based on the solubilization of the mantle at very low ionic strength and neutral pH (0.16 M NaCl and 0.1% NaHCO_3) with 250 ppm of EDTA and further acid precipitation (pH 4.7-4.9) of much of the muscle protein, eliminating the substances responsible for bad taste and odours in the discarded supernatant. Subsequent thermal gelation should be achievable in only one stage, at 90℃, after adding 0.2% Ca(OH)_2 and 1% NaCl. With this processing, gels of about 400 g cm of gel strength can be obttained.
机译:从鱿鱼肌肉加工蛋白质浓缩物(鱼糜)(并进一步凝胶化)的常规方法带来了许多困难,这主要是由于肌原纤维蛋白的特殊性,这会导致广泛的非常高的自溶性,有时还会产生异味和不良现象。肌肉中的味道。为了克服这些问题,已经设计了一种新的程序来处理来自巨型鱿鱼(Dosidicus gigas)肌肉的这种功能性蛋白浓缩物。它是基于在离子强度很低和pH为中性(0.16 M NaCl和0.1%NaHCO_3)的情况下具有非常低的离子强度和250 ppm EDTA的增溶作用以及许多肌肉蛋白的进一步酸沉淀(pH 4.7-4.9),消除了丢弃的上清液中会产生不良味道和气味的物质。加入0.2%Ca(OH)_2和1%NaCl后,在90℃下仅一步可实现随后的热凝胶化。通过这种处理,可以获得约400g·cm的凝胶强度的凝胶。

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