...
首页> 外文期刊>Food Chemistry >Free radical scavenging activity and immunomodulatory effect of Stachytarpheta angustifolia leaf extract
【24h】

Free radical scavenging activity and immunomodulatory effect of Stachytarpheta angustifolia leaf extract

机译:水飞蓟叶提取物的自由基清除活性和免疫调节作用

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       

摘要

Plant extracts with antioxidant activity could also have immunomodulatory ability. The free radical scavenging activity of an ethanol extract of the leaves of Stachytarpheta angustifolia was assessed by measuring its capability for scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anion radical (O_2~(·-)), hydroxyl radical (·OH), nitric oxide radicals (NO·), as well as its ability to inhibit lipid peroxidation, using appropriate assay systems. The extract was also assessed for its ability to decrease the phenotypic expression of the immune activation markers CD38 and CD69. This extract showed a potent antioxidant activity in the DPPH radical-scavenging assay (EC_(50) = 9.65 μg/ml), significantly inhibited OH radical (IC50 = 99.43 μg/ml), O_2~(·-) anion radical (IC50 = 64.68 μg/ml), non-enzymatic lipid peroxidation (IC50 = 282.91 μg/ml) and accumulation of nitrite in vitro. Ex vivo the extract inhibited phorbol myristate acetate (PMA)-induced production of superoxide anion (O_2~(·-), and also exhibited a dose-dependent reduction in the levels of the immune activation marker CD38 and CD69 on phytohemagglutinin A (PHA)-stimulated human peripheral blood mononuclear cells (PBMC). The observed antioxidant activity and immunomodulatory potentials of the extract suggest that it could impart health benefits when consumed. However, further investigation to verify its effect in vivo is warranted.
机译:具有抗氧化活性的植物提取物也可能具有免疫调节能力。通过测量其清除2,2-二苯基-1-吡啶并肼基(DPPH)自由基,超氧阴离子自由基(O_2〜(·-)),羟基的能力,评估了水杉(Stachytarpheta angustifolia)叶片乙醇提取物的自由基清除活性。自由基(·OH),一氧化氮自由基(NO·)以及其抑制脂质过氧化的能力,可以使用适当的测定系统。还评估了提取物降低免疫激活标记CD38和CD69表型表达的能力。该提取物在DPPH自由基清除试验中显示出强大的抗氧化活性(EC_(50)= 9.65μg/ ml),显着抑制了OH自由基(IC50 = 99.43μg/ ml),O_2〜(·-)阴离子自由基(IC50 = 64.68μg/ ml),非酶脂质过氧化(IC50 = 282.91μg/ ml)和亚硝酸盐的体外积累。离体提取物抑制佛波肉豆蔻酸酯乙酸酯(PMA)诱导的超氧阴离子(O_2〜(·-)的产生,并且还显示剂量依赖性降低植物血凝素A(PHA)上免疫激活标记CD38和CD69的水平刺激的人外周血单个核细胞(PBMC),观察到该提取物的抗氧化活性和免疫调节潜能表明该提取物食用后可带来健康益处,但仍需进一步研究以验证其在体内的作用。

著录项

  • 来源
    《Food Chemistry》 |2010年第4期|1409-1416|共8页
  • 作者单位

    Department of Biochemistry, University of Nigeria, Nsukka, Nigeria Department of Chemistry, and Institute for Health & Human Potential, The Global College, University of Winnipeg, Winnipeg, Manitoba, Canada R3B 2E9;

    Department of Biochemistry, University of Nigeria, Nsukka, Nigeria;

    Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada;

    Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada;

    Department of Biochemistry, University of Nigeria, Nsukka, Nigeria;

    Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada;

    Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada;

    Department of Chemistry, and Institute for Health & Human Potential, The Global College, University of Winnipeg, Winnipeg, Manitoba, Canada R3B 2E9;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    stachytarpheta angustifolia extract; free radical scavenging activity; hyperimmune activation;

    机译:水苏花提取物;清除自由基的活性;超免疫激活;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号