Multiresidue method for determination of 88 pesticides in berry fruits using solid-phase extraction and gas chromatography-mass spectrometry: Determination of 88 pesticides in berries using SPE and GC-MS

摘要

A method using solid phase extraction (SPE) cleanup followed by gas chromatography-mass spectrometry (GC-MS) has been established for quantitative determination of 88 pesticide residues in berry fruits including raspberry, strawberry, blueberry and grape. Based on an appraisal of the characteristics of GC-MS, validation experiments were conducted for 88 pesticides. In the method, solid-phase extraction was carried out using Envi-Carb cartridge coupled with NH_2-LC cartridge with acetonitrile-toluene (3:1, v/v) as the eluted solvent. In the linear range of each pesticide, the correlation coefficient was R~2 ≥ 0.99. At the low, medium and high three fortification levels of 0.05-0.5 mg kg~(-1), recoveries fell within 63-137%. The relative standard deviation was between 1% and 19% for all 88 pesticides. Low limits of detection (0.006-0.05 mg kg~(-1)) and quantification (0.02-0.15 mg kg~(-1)) were readily achieved with this method for all tested pesticides.