Chemical constituents, antioxidant and gastrointestinal transit accelerating activities of dried fruit of Crataegus dahurica

摘要

Crataegus dahurica Koehne is an edible wild fruit mainly distributed in Northeast China. The purpose of this study was to elucidate the chemical constituents and investigate the bioactivities of dried fruit of C. dahurica methanol extract (CdME). Through various chromatographic methods, thirty-five compounds were isolated from CdME for the first time and their structures were identified on the basis of physicochemical properties and spectroscopic data. The main structural types of these compounds were triterpenoids and polyphenolics. Pharmacological experiments results showed that CdME had potently antioxidant capacity and ethyl acetate fraction was the active part with the greatest antioxidant activities. Moreover, CdME especially n-butanol fraction significantly accelerated the gastrointestinal transit in mice (acceleration rate: 78.5 +/- 1.5% vs. 69.9 +/- 3.2% at a dose of 250 mg/kg, compared to the control group, P .01). On the basis of these results, C. dahurica may be considered as a good resource of antioxidants and digestion-improving agents.