Recovery of bioactive and gelling extracts from edible brown seaweed Laminaria ochroleuca by non-isothermal autohydrolysis

摘要

The non-isothermal autohydrolysis temperature impact of edible brown seaweed Laminaria ochroleuca was studied to recover high valuable compounds. Extraction yield was determined, above 80% was obtained at 220 degrees C. The maximal fucose content (17% d.b.) was attained at 180 degrees C, whereas the maximal sulphate was achieved at 160 degrees C, and phenolic and protein content at 220 degrees C. The maximum sulphated fucoidan content (41.38 g fucoidan/100g extract) was obtained at 160 degrees C, whereas the maximum fucose oligosaccharides was obtained at 180 degrees C. The antioxidant capacity was equivalent to 32 mg Trolox/g dry extract produced at 220 degrees C. The milder processing condition was selected to study the potentiality of the precipitated alginate in terms of viscoelastic properties determined by rheology. Alginate extraction (14.94 g/100 g extract) was determined at 160 degrees C. The crude fucoidan fractions were tested at 25-500 mu g/mL, showed up to 50% cell growth inhibition in four selected tumoral cell lines.