Drop-Type Chemiluminescence (DCL) System and Sample Treatment Platform Using Magnetic Nanoparticles to Determine Enrofloxacin and Its Metabolite in a Chicken Meat

摘要

An analytical method to determine antibiotics using a lab-built drop-type chemiluminescence (DCL) system is introduced. Magnetic nanoparticles (MNPs) functionalized with amine groups were used for the extraction of antibiotics from a pretreated sample solution by magnetic separation. Detection is achieved with an antibody specific against enrofloxacin and ciprofloxacin and a secondary horseradish peroxidase (HRP)-labelled antibody. The enzyme is capable of catalyzing the luminol reaction, which can be read out with the DCL device. For application, the DCL system was used to determine enrofloxacin and its metabolite in chicken meat. The obtained detection limits were 0.039 pmol/mL (~0.12 ng/mL) and 0.022 pmol/mL for enrofloxacin and ciprofloxacin, respectively, which are about 10 times better than enzyme-linked immunosorbent assay (ELISA).