Aflatoxin M_1 and cyclopiazonic acid in fluid milk traded in Sao Paulo, Brazil

摘要

Aflatoxin M_1 (AFM_1) and cyclopiazonic acid (CPA) were determined in 48 samples of grade A-C and ultra-high-temperature (UHT)-treated fluid commercial milk from Sao Paulo, Brazil, from July to December 2004. AFM_1 and CPA determinations were carried out by HPLC. Results showed 37 (77.1%) positive samples for AFM_1 at levels of 0.011-0.251 μg l~(-1) milk, which were below the tolerance limit for AFA_1 in milk as adopted by Brazilian regulations. Mean levels of AFM_1 in grades A-C and UHT milk were 0.060 ±0.080, 0.056 ±0.063, 0.064 ±0.041 and 0.075 ±0.048 μg l~(-1), respectively. CPA was detected in two samples (4.2%) of grade A milk at levels of 6.4 and 9.7 μg l~(-1). This is the first report of the occurrence of CPA in milk in Brazil. It was concluded that the incidence of AFM_1 in milk traded in Sao Paulo is low, but the simultaneous occurrence of CPA, even at low levels, warrants concern about AFM_1 in milk and milk products.