Interference of anthocyanin extracted from black soybean coats on aflatoxin B1-human serum albumin in the binding process

摘要

The effect of the anthocyanin cyanidin-3-O-glucoside (C3G) and its main gastrointestinal metabolites (PCA and PGA) on the binding of AFB_1 and HSA were studied via spectrometry. C3G is relatively stable in the gastric environment, and the intestinal environment promotes its metabolism into PCA and PGA. Binary fluorescence experiments showed that both AFB_1 and C3G, including PCA and PGA, can react with HSA. AFB_1 C3G and PCA can bind at site Ⅰ and site Ⅱ of HSA; PGA binds at site Ⅱ. The presence of C3G/PCA/PGA inhibits the degree of quenching. C3G/PCA does not change the quenching mechanism; it is still static quenching; however, dynamic quenching occurs in the (AFB_1-HSA)-PGA system. In addition, the apparent binding constant and number of binding sites of AFB_1-HSA also diminish to different degrees. C3G and its metabolites (PCA and PGA) interfere with the interaction between AFB_1 and HSA, and can reduce AFB_1 transport at pH 7.4 in vitro.