首页> 外文期刊>Environmental Toxicology and Chemistry >PERSISTENCE AND DEGRADATION OF MAIZE-EXPRESSED VACCINE PROTEIN, ESCHERICHIA COLI HEAT-LABILE ENTEROTOXIN SUBUNIT B, IN SOIL AND WATER
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PERSISTENCE AND DEGRADATION OF MAIZE-EXPRESSED VACCINE PROTEIN, ESCHERICHIA COLI HEAT-LABILE ENTEROTOXIN SUBUNIT B, IN SOIL AND WATER

机译:在土壤和水中玉米表达的疫苗蛋白,大肠埃希氏大肠杆菌热肠毒素B的持久性和降解

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摘要

Transgenic plants represent an innovative platform for the cost-effective large-scale production of various pharmaceutical proteins. The eventual open-field production of plant-made pharmaceuticals (PMPs) requires risk assessment to determine the potential for harm to the surrounding ecosystem. In the present study, the environmental persistence of a transgenic maize-expressed antigen, Escherichia coli heat-labile enterotoxin subunit B (LTB), was studied under laboratory conditions. To semiquantitatively monitor the persistence of LTB in soil, extraction with a high-salt, high-pH extraction buffer was optimized using the closely homologous Vibrio cholerae enterotoxin subunit B (CTB) as a test substance. The time to dissipation of 50% (DT50) of the extractable fraction of maize-expressed LTB was 4 to 15 d in pond water and 35 to 90 d in soils. Both extraction efficacy and persistence were strongly affected by the matrix type and incubation conditions. In contrast with maize-expressed LTB, the DT50 for bacterially produced LTB and CTB was less than 4 d both in pond water and soil. Although maize-expressed LTB was more stable than bacterially produced analogue, its dissipation was governed by an initial lag, which could be attributed to release from the plant material, followed by rapid decline.
机译:转基因植物代表了一个创新的平台,可以经济高效地大规模生产各种药物蛋白。最终开放生产的工厂生产的药物(PMP)需要进行风险评估,以确定对周围生态系统的潜在危害。在本研究中,在实验室条件下研究了转基因玉米表达的抗原,大肠杆菌热不稳定肠毒素亚单位B(LTB)的环境持久性。为了半定量监测土壤中LTB的持久性,使用紧密同源的霍乱弧菌肠毒素亚单位B(CTB)作为测试物质优化了高盐,高pH提取缓冲液的提取。玉米表达的LTB的可提取部分的50%(DT50)消散时间在池塘水中为4至15 d,在土壤中为35至90 d。提取效率和持久性都受到基质类型和孵育条件的强烈影响。与玉米表达的LTB相反,在池塘水和土壤中,细菌产生的LTB和CTB的DT50均小于4 d。尽管玉米表达的LTB比细菌产生的类似物更稳定,但其耗散受初始滞后的控制,这可能是由于从植物材料中释放出来,然后迅速下降。

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