AN IN VITRO ASSESSMENT OF THE TOXICITY OF 2,4-DICHLOROANILINE USING RAINBOW TROUT PRIMARY EPIDERMAL CELL CULTURES

摘要

2,4,-Dichloroaniline(2,4,-DCA)is a common pollutant of freshwaters. Information on the effects of this chemical on the aquatic environment is lacking. In this work, we present a technique using primary cell culture of rainbow trout(Oncorhynchus mykiss)epidermal cells as a model for the evaluation of the potential ecotoxicologic hazards of 2,4-DCA. The effects of a wide dose range of the chemical on survival and function of the cells were studied. 2,4-Dichloroaniline was found to show an increasingly toxic effect over the dose range of 100 to 1,000 Μm.