Comparative Die-off of Escherichia coli 0157:H7 and Fecal Indicator Bacteria in Pond Water

摘要

In situ and in vitro experiments were performed to assess the effects of solar radiation and predab'on by indigenous microflora on the relative die-off rates of a toxigenic strain of Escherichia coli 0157:H7, commensal £ coli, and fecal enterococci in surface waters from ponds in agricultural watersheds. The objective of these experiments was to discern a mechanism of persistence of £ coli 0157:H7 in surface waters compared to fecal indicator bacteria. Results of these experiments indicated that £ coli and fecal enterococci were affected by both insolation and apparent predation; whereas E. coli 0157:H7 appeared to be resistant to both of these environmental stressors. The number of days to reach 99% die-off (T_(99)-values) for £ coli 0157:H7 was significantly greater than that for the indicator bacteria. The capacity to prolong die-off may be connected to the apparent persistence of £ coli 01S7:H7 in surface waters.