Adhesion of Vibrio parahaemolyticus to Stainless Steel Surfaces and Its Removal by Caustic Alkali Cleaning

摘要

The adhesion of V. parahaemolyticus to stainless steel surfaces and its removal by caustic alkali cleaning were studied as a function of pH. The surface charge property of V. parahaemolyticus cells could be characterized successfully by potentiometric titration. The apparent point of zero charge (pzc_(app)) of V. parahaemolyticus cells was found to be 6.1. V. parahaemolyticus cells could adhere irreversibly to stainless steel surfaces over the pH range of 3 to 10. The isotherm for cell adhesion at pH 6.1 showed saturation curve, like as Langmuir-type one, giving saturation value of approximately 7.0 mg dry cells/m~2. The removal of V. parahaemolyticus increased considerably depending on the concentration of OH~-.%ステンレス鋼表面へのVibrio parahaemolyticusの付着と苛性アルカリ洗浄による除去についてpHの関数として検討した。V. parahaemolyticusの表面電荷特性は、電位差滴定法によりうまく測定することができた。V. parahaemolyticusの見掛けの零電荷点(pzc_(app))は6.1であった。V. parahaemolyticusは、pH 3〜10の範囲でステンレス鋼表面に不可逆的に付着することができた。pH 6.1での付着等温線はラングミュア型の飽和曲線を示し、飽和値は約7.0mg dry cells/m~2であった。V. parahaemolyticusの除去率は、OH~-濃度に依存して大幅に増加した。