Mitigation of arsenic toxicity and accumulation in hydroponically grown rice seedlings by co-inoculation with arsenite-oxidizing and cadmium- tolerant bacteria

摘要

Arsenic (As) contamination of rice grain is a serious problem worldwide. The objective of this study was to mitigate As toxicity and accumulation in hydroponically grown KDML105 rice seedlings using bacteria isolated from heavy metal-contaminated soils. Seven strains (KKU2500-1, -2, -3, -9, -12, -16 and -22) of 24 cadmium (Cd)-tolerant bacteria produced high levels of inorganic sulfide and thiol-rich compounds in As-supplemented media. The strains were allowed to colonize rice seedlings growing in arsenite [As(III)]- or arsenate [As(V)]-supplemented Hoagland's nutrient solutions. Colonization by strains KKU2500-3 and - 12 led to increases in plant growth parameters and similarly reduced As translocation into shoots [translocation factor (TF) = 0.05] in the As(V)-supplemented solution. Strains KKU2500-1 and - 12 also greatly reduced As translocation into shoots (TF = 0.16-0.20) in As(III)-supplemented solution. KKU2500-3 and - 12 co-colonized onto seedlings with the As(III)-oxidizing isolates 4.25, 4.27, 4.40 and 4.44, and the strain combinations KKU2500-12/4.25, KKU2500-3/4.25, KKU2500-3/4.27 and KKU2500-3/4.44 resulted in higher growth parameters for plants grown in As [As(III) + As(V)]-supplemented solution than other combinations. Moreover, the combinations KKU2500-3/4.25 and KKU2500-3/4.44 greatly reduced As translocation (TF = 0.15 and 0.12, respectively), and this decreased As accumulation in shoots was significantly correlated with increased sulfide stimulation in roots and nutrient solution. These results indicate that these co-inoculated bacteria can mitigate As toxicity, translocation and accumulation in ICDML105 seedlings and thus demonstrate synergistic activity in rice plants, and this effect can be further developed in field trials.