Original Articles: Tumor Necrosis Factor-α Acutely Inhibis insulin Signaling in Human Adipocytes

摘要

Tumor necrosis factor(TNF)-αis postulated to play a major role in the pathogenesis of obesity-linked insulin resistance, probably resulting from an interaction with insulin signaling pathways. This cross talk has now been investigated in human adipocytes at the level of phyosphatidylinositol (PI) 3-kinase, and the TNF recep- tors (TNFRs) mediating these processes have been identified. Equilibrium binding studies using human adipocytes from mammary tissue indicated the pres- ence of two populations of TNFR with apparent affin- ity constants of 13j pmol/l and 1.6 nmol/l, respectively.