In vitro evaluation of different nanoparticles and green synthesized nano pesticides against Streptomyces scabies causing the disease “common scab of potato”

摘要

Potato (Streptomyces scabies) is thought to be a common and economically important disease of potato worldwide. It is most important kitchen item which is cooked as vegetable. Potato crop is affected by so many diseases, but bacterial diseases are more common. In this context an environment friendly approach of using green synthesis nanoparticles to manage this pathogen was used. In this study, disease causing bacteria was isolated from infected potatoes. Bacteria was purified and identified under microscope on the base on morphological characteristics. In the context of disease management, the pathogen was treated with different green synthesis nanoparticles. Common scab of potato produced by Streptomyces scabies is among one of the destructive diseases of potato in Pakistan. The market value and quality of potatoes are reduced by this disease. The current study was conducted out to evaluate the effectiveness of different nano particles and green synthesized nano pesticides against Streptomyces scabies. In vitro experiments were laid down in complete randomized design (CRD) and the inhibition zone technique was used for the evaluation of nano pesticides. The results of the analysis of variance demonstrated a substantial difference between the different therapies for limiting bacterial growth against disease under disease pressure. After 72 hours, the mean comparisons revealed that single and consortium administration of nanoparticles and green manufactured nano insecticides inhibited S. scabies (mm). The combination of ZnO+Streptomycin injection (27.930 mm) followed by Streptomycin injection therapy exhibited the lowest mean comparison of bacterial growth (28.330 mm). The mean comparison showed that the maximum bacterial growth in control (38.330) at all concentrations of 25ppm, 50ppm and 75ppm respectively. The results were most effective at concentration at 75ppm for 72 hours. The combination of ZnO+Streptomycin injection was the most effective among all the treatments against S. scabies. It is concluded that the results of this research will help to combat the common scab disease of potatoes and enhance potato crop exports.