HISTOPATHOLOGICAL EXAMINATION OF THE EFFECT OF BLACK SEED (Nigella sativa) OIL ON THE METHOTREXATE-INDUCED GASTROINTESTINAL MUCOSA INJURY AND HEPATOTOXICITYIN MICE

摘要

Black seed(Nigella sativa) oil is one of the herbal product which used for therapeutic purposes. We aimed to investigate the effects ofblack seed oil on irritated gastrointestinal mucosa and liver. 21 healthy swiss albino mice were randomly divided into three groups: control group,group-2, group-3. The MTX(20 mg/kg)was given intraperitoneally as a single doseon the lst day to the group-2 and group-3.Black seed oil(4 ml/kg) was administered by ga-vages into the stomach daily for7 days to the group-3. No pathology was observed in control group. In gastric mucosa histopathology, no pathology was ob-served in the gastric mucosa of group-2 and group-3. In the histopathological examination of the intestinal mucosa, significant hyperplasia and mononuclear cell infiltrations were observed in the intestines of the mice forming the group-2, characterized by an increase in villous epithelial cells. In the intestines of group-3, marked hyperplasia was observed,charac-terized by an increase in villous epithelial cells, and no other pathognomic findings in group-2 were ob-served. In liver histopathology, vascular congestion, sinusoidal enlargement, mononuclear cell infiltra-tions in periportal areas and local degenerative changes were observed in group-2 and group-3.Ac-cording to the data we obtained in our study, black seed oil has no irritating side for stomach and intes-tinal mucosa, and it was also seen that it has a pro-tective and therapeutic effect. However, it was also observed in this study that the black seed did not af-fect the damaged liver and did not have a healing ef-fect.