Effect and mechanism of CyPA in radiosensitization of lung adenocarcinoma using CRISPR/Cas9 technology

摘要

ObjectiveTo explore the role of cyclophilin A (CyPA) in sensitization of lung adenocarcinoma to radiotherapy using CRISPR/Cas9 technology.MethodsA CyPA knockout human lung adenocarcinoma cell line H1975 was established by CRISPR/Cas9 technology. Groups included a control group (wildtype), CyPA knockout group, traditional Chinese medicine (TCM) extract with Fuzhengzengxiao decoction group, and TCM extract with Fuzhengzengxiao decoction?+?CyPA knockout group. Each group was exposed to radiation at doses of 0, 2, 4, 6, and 8?Gy. After 24?h, MTT assays were used to determine the survival rate of lung cancer cells and calculate radiosensitivity. The qPCR was used to measure mRNA expression of DDIT3, CDKN1A, and CDC25A associated with DNA damage repair.ResultsWithout irradiation, Fuzhengzengxiao decoction reduced the survival rate of lung adenocarcinoma cells (P?