Antidiabetic, antioxidant, molecular docking and HPTLC analysis of miquelianin isolated from Euphorbia schimperi C. Presl

摘要

The present study demonstrates the miquelianin or quercetin 3- O -glucuronide (compound 1 ) isolated from aerial parts of Euphorbia schimperi exhibited significant results for antioxidant and antidiabetic potential. The compound 1 along with kaempferol 3- O -glucuronide (compound 2 ) and quercetin 3- O -rhamnoside (compound 3 ) isolated from the same source were quantified by validated HPTLC method. Antioxidant activity was determined by chemical means in terms of ABTS radical cation and DPPH radical scavenging activity. Compound 1 showed significant scavenging activity in both ABTS and DPPH assays as compared to standard BHA. In ABTS method ICsub50/sub values of compound 1 and standard BHA is found to be 58.90?±?3.40?μg/mL and 28.70?±?5.20?μg/mL respectively while in DPPH assay ICsub50/sub values of Compound 1 and standard BHA is 47.20?±?4.90?μg/mL and 34.50?±?6.20?μg/mL respectively. Antidiabetic effect was studied through α-amylase and α-glucosidase inhibitory activity. The mechanistic approach through molecular modelling also support the strong binding sites of compound 1 which showed significant α-amylase and α-glucosidase inhibitory activities with ICsub50/sub values 128.34?±?12.30 and 89.20?±?9.20?μg/mL respectively as compared to acarbose 64.20?±?5.60 and 52.40?±?4.60?μg/mL respectively. The results of validated RP-HPTLC analyses revealed the concentration of compound 1 found to be 16.39?μg/mg and for compound 2 and compound 3 as 3.92 and 14.98?μg/mg of dried extract, respectively.