Study on rapid detection of seven common foodborne pathogens by gene chip

摘要

To develop a rapid, effective, specific, and sensitive method to detect foodborne pathogens, 13 sets of primers were designed to amplify the conservative and specific genes of rfbE, fliC, invA, hilA, ipaH, femA, nuc, hlyA, prfA, tuf, speB, tlh and tdh, respectively. Establishment of foodborne pathogens detection chips was conducted by spotting the target genes on the chips by Nano-PlotterTM NP 1.2 printing system. The DNA of 7 standard pathogenic strains and 147 strains extracts from food samples was amplified and labeled for hybridization. The results demonstrated that enterhemorrhagic Escherichia coli O157:H7, Salmonella enteritidis, Shigella flexner, Staphylococcus aureus, Listeria monocytogenes, β-hemolytic streptococcus, and Vibrio parahaemolyticus could correctly be identified by the designed gene chip at an optimal temperature of 58°C and were proved as a potential method with good stability and sensitivity (5 pg/μl of template DNA).