Detecting the Y chromosome with C-banding and chromosome Painting technique in the mandarin vole Microtus mandarinus

摘要

The Y chromosome of Microtus mandarinus was detected with the C-banding technique and whole Y chromosome specific probe. Two C-bands on X chromosome were distinguished ,a distinct one on the kinetochore, and a pale one on the short arm in the metaphase chromosome of the male Microtus mandarinus . We did not find the Y-chromosome which was dyed deeply. Metaphase chromosome and interphase nucleus of marrow cells of Microtus mandarinus were painted with the whole Y chromosome specific probe of mouse , and the metaphase chromosome and interphase nucleus of mouse marrow cells was used as the control . The painting results showed that the signal detection rate is about 0 to 2% in the metaphase chromosomes and 3% to 5% in the interphase nucleus of marrow cell of Microtus mandarinus , and the results are negative, but the control experiment was positive. From the results, we deduce that there are no homologic sequences of whole Y chromosome special DNA sequences of mouse in Y chromosome and whole genome of Microtus mandarinus . Perhaps, there are no important genes which determine male sex in the Y chromosome [ Acta Zoologica Sinica 50 (2): 245 - 251 , 2004].