DOP (degenerate oligonucleotide primer)-PCR amplification of a chromosome in a method for comparing chromosomes from two different cells of different karyotypes for detecting chromosomal abnormalities using CGH and micro arrays

摘要

Disclosed is a method of comparing at least one chromosome or part thereof from a cell with a first karyotype with the corresponding chromosome or part thereof from a cell with a second karyotype, the method including: (a) amplifying DNA from an isolated chromosome or part of an isolated chromosome, the amplified DNA being depleted of repetitive sequences; (b) attaching the amplified DNA to a solid substrate; (c) amplifying DNA from one or more cells with a first karyotype and amplifying DNA from one or more cells with a second karyotype; (d) labelling the amplified DNA from the one or more cells with a first karyotype with a first label, and labelling the amplified DNA from the one or more cells with a second karyotype with a second label, wherein the first and second labels are detectably different; (e) hybridizing the amplified and labelled DNA from the one or more cells with a first karyotype to the amplified DNA attached to the solid substrate, and hybridizing the amplified and labelled DNA from the one or more cells with a second karyotype to the amplified DNA attached to the solid substrate; and (f) comparing the relative amount of first and second labels hybridized to the amplified DNA attached to the solid substrate and thereby compare at least one chromosome or part thereof from the cell with a first karyotype with the corresponding chromosome or part thereof from the cell with a second karyotype.