Detection of Human Metapneumovirus in Clinical Samples by Immunofluorescence Staining of Shell Vial Centrifugation Cultures Prepared from Three Different Cell Lines

Marie L. Landry; David Ferguson; Sandra Cohen; Teresa C. T. Peret; Dean D. Erdman

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Detection of Human Metapneumovirus in Clinical Samples by Immunofluorescence Staining of Shell Vial Centrifugation Cultures Prepared from Three Different Cell Lines

机译：通过三种不同细胞系制备的壳病毒离心培养物的免疫荧光染色检测临床样本中的人类偏肺病毒

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Monoclonal antibody MAb-8 was evaluated for detection of human metapneumovirus (HMPV) in shell vial centrifugation cultures (SVCC). Detection of HMPV was similar in A549, HEp-2, and LLC-MK2 SVCC, and MAb-8 staining was optimal on day 2 postinoculation. Availability of SVCC for HMPV will be of significant benefit to clinical laboratories.

机译：评估了单克隆抗体MAb-8在壳小瓶离心培养物中（SVCC）中人间肺炎病毒（HMPV）的检测。 HMPV的检测在A549，HEp-2和LLC-MK2 SVCC中相似，并且接种后第2天MAb-8染色最佳。 SVCC可用于HMPV，将对临床实验室带来重大好处。

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《Journal of Clinical Microbiology》 |2005年第4期|共3页
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Marie L. Landry; David Ferguson; Sandra Cohen; Teresa C. T. Peret; Dean D. Erdman;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Comparison of Chemicon SimulFluor Direct Fluorescent Antibody Staining with Cell Culture and Shell Vial Direct Immunoperoxidase Staining for Detection of Herpes Simplex Virus and with Cytospin Direct Immunofluorescence Staining for Detection of Varicella-Zoster Virus [J] . Edward L. Chan, Ken Brandt, Greg B. Horsman Clinical and diagnostic laboratory immunology . 2001,第5期

机译：Chemicon SimulFluor直接荧光抗体染色与细胞培养和壳管直接免疫过氧化物酶染色用于检测单纯疱疹病毒以及与Cytospin直接免疫荧光染色用于检测水痘-带状疱疹病毒的比较
2. Comparison of different cell lines and incubation times in the isolation by the shell vial culture of human metapneumovirus from pediatric respiratory samples. [J] . Reina J, Ferres F, Alcoceba E, Journal of clinical virology: The official publication of the Pan American Society for Clinical Virology . 2007,第1期

机译：壳小瓶培养分离人小儿肺炎病毒的小细胞呼吸道样品中不同细胞系和孵育时间的比较。
3. Detection of human metapneumovirus by direct antigen test and shell vial cultures using immunofluorescent antibody staining. [J] . Jun KR, Woo YD, Sung H, Journal of Virological Methods . 2008,第1a2期

机译：通过直接抗原测试和使用免疫荧光抗体染色的壳管培养物检测人偏肺病毒。
4. Targeted Proteomics of Human Metapneumovirus in Clinical Samples and Viral Cultures [C] . Matthew W. Foster, Geoff Gerhardt, Lynda Robitaille, ASMS Conference on Mass Spectrometry and Allied Topics . 2015

机译：临床样品和病毒培养中人体孢子虫病毒的靶向蛋白质组学
5. Determining the relationship of human enteric viruses in clinical, wastewater, and environmental samples utilizing molecular and cell culture techniques. [D] . Woods, Jacquelina Susann. 2010

机译：利用分子和细胞培养技术确定临床，废水和环境样品中人类肠道病毒的关系。
6. Detection of Human Metapneumovirus in Clinical Samples by Immunofluorescence Staining of Shell Vial Centrifugation Cultures Prepared from Three Different Cell Lines [O] . Marie L. Landry, David Ferguson, Sandra Cohen, 2005

机译：通过三种不同细胞系制备的壳病毒离心培养物的免疫荧光染色检测临床样品中的人类偏肺病毒
7. Detection of Human Metapneumovirus in Clinical Samples by Immunofluorescence Staining of Shell Vial Centrifugation Cultures Prepared from Three Different Cell Lines [O] . Landry, Marie L., Ferguson, David, Cohen, Sandra, 2005

机译：通过三种不同细胞系制备的壳病毒离心培养物的免疫荧光染色检测临床样品中的人类偏肺病毒
8. Application of Modified Shell Vial Culture Procedure for Arbovirus Detection [R] . Caceda, E. R., Kochel, T. J. 2007

机译：改良壳小瓶培养法在虫媒病毒检测中的应用

Detection of Human Metapneumovirus in Clinical Samples by Immunofluorescence Staining of Shell Vial Centrifugation Cultures Prepared from Three Different Cell Lines

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