Prospective Study of Human Metapneumovirus Detection in Clinical Samples by Use of Light Diagnostics Direct Immunofluorescence Reagent and Real-Time PCR

摘要

A Light Diagnostics human metapneumovirus (HMPV) monoclonal antibody reagent was evaluated using cytospin-enhanced direct immunofluorescence (DFA), and the results were compared to those for TaqMan reverse transcription-PCR (RT-PCR). Of the 202 samples tested, 48 were positive by RT-PCR, and 41 (85.4%) of these were positive by DFA (P = 0.0771). The commercial availability of an HMPV DFA reagent will be of significant benefit to clinical laboratories.