Biochemical Identification ofCitrobacter Species Defined by DNA Hybridization and Description of Citrobacter gillenii sp. nov. (FormerlyCitrobacter Genomospecies 10) and Citrobacter murliniae sp. nov. (Formerly CitrobacterGenomospecies 11)

摘要

Recent work describing six named species and two unnamed genomospecies within Citrobacter has enlarged the genus to 11 species. DNA relatedness and phenotypic tests were used to determine how well these species can be identified. One hundred thirty-six strains were identified to species level by DNA relatedness and then identified phenotypically in a blinded fashion. By using conventional tests, 119 of the 136 strains (88%) were correctly identified to species level. Three additional strains (2%) were identified as citrobacteria but were not identified to species level, and 14 strains (10%) were misidentified as other Citrobacter species. Carbon source utilization tests were used to identify 86 of the strains. Eighty-four strains (98%) were correctly identified, and two strains (2%) were misidentified as other Citrobacterspecies. Additional strains of Citrobacter genomospecies 10 and Citrobacter genomospecies 11 were identified, allowing these species to be formally named as Citrobacter gilleniisp. nov. and Citrobacter murliniae sp. nov., respectively.