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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of lipopolysaccharide and outer membrane protein-lipopolysaccharide extracts in an enzyme-linked immunosorbent assay for the diagnosis of Brucella ovis infection.
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Comparison of lipopolysaccharide and outer membrane protein-lipopolysaccharide extracts in an enzyme-linked immunosorbent assay for the diagnosis of Brucella ovis infection.

机译:酶联免疫吸附法中脂多糖和外膜蛋白-脂多糖提取物的比较,用于诊断猪布鲁氏菌感染。

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摘要

Brucella ovis hot saline extracts and petroleum ether-chloroform-phenol lipopolysaccharide were compared in an enzyme-linked immunosorbent assay for the diagnosis of B. ovis ram epididymitis. Hot saline extracts detected greater numbers of infected rams. Chemical characterization of the antigens showed that, although both contained lipopolysaccharide, hot saline extracts also contained outer membrane proteins. These proteins were active as antigens in Western blot tests with sera of infected rams, and therefore they explained the better diagnostic results obtained with hot saline extracts. However, compared with lipopolysaccharide, hot saline extracts showed a higher degree of cross-reactivity with sera from smooth B. melitensis-infected animals. This observation might be explained by the presence of B. ovis outer membrane proteins in hot saline extracts which lack the specificity necessary for serological identification of the Brucella species present.
机译:在酶联免疫吸附试验中比较了布鲁氏菌羊热盐提取物和石油醚-氯仿-苯酚脂多糖,以诊断牛芽孢杆菌附睾炎。热盐水提取物检测到大量受感染的公羊。抗原的化学特征表明,尽管两者都含有脂多糖,但是热盐水提取物也含有外膜蛋白。这些蛋白在被感染公羊血清的蛋白质印迹试验中作为抗原具有活性,因此它们解释了用热盐水提取物可获得更好的诊断结果。然而,与脂多糖相比,热食盐水提取物与光滑的B. melitensis感染动物的血清显示出更高的交叉反应性。这种观察可能是由于在热盐水提取物中存在牛肝芽孢杆菌外膜蛋白而缺乏血清学鉴定存在的布鲁氏菌种所必需的特异性。

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