C. elegans RNA-binding proteins PUF-8 and MEX-3 function redundantly to promote germline stem cell mitosis

Mohd Ariz; Rana Mainpal; Kuppuswamy Subramaniam

首页> 外文期刊>Developmental biology >C. elegans RNA-binding proteins PUF-8 and MEX-3 function redundantly to promote germline stem cell mitosis

【24h】

C. elegans RNA-binding proteins PUF-8 and MEX-3 function redundantly to promote germline stem cell mitosis

机译：秀丽隐杆线虫RNA结合蛋白PUF-8和MEX-3多余地促进种系干细胞的有丝分裂

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Maintenanceofmitoticallycyclinggermlinestemcells(GSCs)isvitalforcontinuousproductionofgametes.Inwormsandinsects,signalingfromsurroundingsomaticcellsplayanessentialroleinthemaintenanceofGSCsbypreventingprematuredifferentiation.Inaddition,germcellproteinssuchastheemDrosophila/emPumilioandemCaenorhabditiselegans/emFBF,bothmembersofthePUFfamilytranslationalregulators,contributetoGSCmaintenance.FBFfunctionsbysuppressingGLD-1,whichpromotesmeioticentry.However,factorsthatdirectlypromoteGSCproliferation,ratherthanpreventdifferentiation,arenotknown.HereweshowthatPUF-8,anotheremC.elegans/emmemberofthePUFfamilyandMEX-3,aKHdomaintranslationalregulator,functionredundantlytopromoteGSCmitosis.WefindthatPUF-8proteinishighlyenrichedinmitoticgermcells,whichissimilartotheexpressionpatternofMEX-3describedearlier.Theempuf-8(minus;)mex-3(minus;)/emdoublemutantgonadscontainfarfewergermcellsthanbothsinglemutantsandwild-type.Whilethesecellslackmitotic,meioticandspermmarkers,theyretainthegermcell-specificPgranules,andarecapableofgametogenesisifGLP-1,whichnormallyblocksmeioticentry,isremoved.Significantly,wefindthatatleastoneofthesetwoproteinsisessentialforgermcellproliferationeveninmeioticentry-defectivemutants,whichotherwiseproducegermcelltumors.WeconcludePUF-8andMEX-3contributetoGSCmaintenancebypromotingmitoticproliferationratherthanbyblockingmeioticentry./p/div

机译：Maintenanceofmitoticallycyclinggermlinestemcells（GSC中）isvitalforcontinuousproductionofgametes.Inwormsandinsects，signalingfromsurroundingsomaticcellsplayanessentialroleinthemaintenanceofGSCsbypreventingprematuredifferentiation.Inaddition，germcellproteinssuchasthe 果蝇 Pumilioand Caenorhabditiselegans FBF，bothmembersofthePUFfamilytranslationalregulators，contributetoGSCmaintenance.FBFfunctionsbysuppressingGLD-1，whichpromotesmeioticentry.However，factorsthatdirectlypromoteGSCproliferation，ratherthanpreventdifferentiation，arenotknown。我们在此显示PUF家族的另一个 C.elegans 成员和一个KH域翻译调节子MEX-3，其冗余冗余地导致了GS丝裂。 / em>双突变性腺包含比单个突变体和野生型少的生殖细胞。而这些细胞缺乏有丝分裂，减数分裂和精子标记，它们保留了生殖细胞去除了通常能阻止减数分裂进入的L-特异性颗粒，其具有可阻止配子发生的作用。重要的是，我们确定这两个蛋白质缺陷型生殖细胞缺陷的突变体甚至减数分裂突变体，否则通过减数分裂而抑制了生殖细胞。我们通过延长寿命来抑制PUF-8和M-3。 展开▼

著录项

来源
《Developmental biology》 |2009年第2期|共页

作者
Mohd Ariz; Rana Mainpal; Kuppuswamy Subramaniam;
展开▼

作者单位

展开▼

收录信息

原文格式 PDF

正文语种

中图分类生物科学;

关键词

相似文献

外文文献

中文文献

专利

1. C. elegans RNA-binding proteins PUF-8 and MEX-3 function redundantly to promote germline stem cell mitosis. [J] . Ariz M, Mainpal R, Subramaniam K Developmental biology . 2009,第2期

机译：秀丽隐杆线虫RNA结合蛋白PUF-8和MEX-3具有多余的功能，以促进种系干细胞的有丝分裂。

2. PUF-8 suppresses the somatic transcription factor PAL-1 expression in C. elegans germline stem cells. [J] . Mainpal R, Priti A, Subramaniam K Developmental biology . 2011,第1期

机译：PUF-8抑制线虫种系干细胞中的体细胞转录因子PAL-1表达。

3. PUF-8 suppresses the somatic transcription factor PAL-1 expression in C. elegans germline stem cells [J] . Rana Mainpal, Agarwal Priti, Kuppuswamy Subramaniam Developmental biology . 2011,第1期

机译：PUF-8抑制线虫种系干细胞中的体细胞转录因子PAL-1表达

4. Investigating Spatio-Temporal Cellular Interactions in Embryonic Morphogenesis by 4D Nucleus Tracking and Systematic Comparative Analysis — Taking Nematodes C. Elegans and C. Briggsae as Examples [C] . Guoye Guan, Wong Ming-Kin, Chan Lu-Yan, IEEE International Conference on Bioinformatics and Computational Biology . 2021

机译：通过4D核跟踪和系统对比分析研究胚胎形态发生中的时空细胞相互作用 - 以Nematode C.Egrigans和C. Briggsae为例

5. Stem cells and differentiation in the C. elegans male germline [D] . Morgan, Dyan Elisabeth 2011

机译：秀丽隐杆线虫男性生殖系中的干细胞及其分化

6. C. elegans RNA-binding proteins PUF-8 and MEX-3 function redundantly to promote germline stem cell mitosis [O] . Mohd Ariz, Rana Mainpal, Kuppuswamy Subramaniam -1

机译：秀丽隐杆线虫RNA结合蛋白PUF-8和MEX-3冗余地促进种系干细胞的有丝分裂

7. C. elegans RNA-binding proteins PUF-8 and MEX-3 function redundantly to promote germline stem cell mitosis [O] . Ariz, Mohd, Mainpal, Rana, Subramaniam, Kuppuswamy 2009

机译：秀丽隐杆线虫RNA结合蛋白PUF-8和MEX-3冗余地促进种系干细胞的有丝分裂

1. Mex-3 RNA结合蛋白在肿瘤中的研究进展 [J] . 罗祥 ,黄雯琪 . 重庆医学 . 2021,第020期

2. 环状RNA circ0025847通过促进RNA结合蛋白QK1表达抑制结直肠癌细胞的增殖 [J] . 龚磊 ,冯雪雅 ,吴洪 . 中华内分泌外科杂志 . 2021,第005期

3. 张进研究员团队揭示核仁定位的RNA结合蛋白LIN28调控小鼠胚胎干细胞多能性状态与二细胞样状态转变机制 [J] . . 浙江大学学报（医学版） . 2021,第004期

4. 多能干细胞关键因子Oct4 RNA结合蛋白的分离与鉴定 [J] . MA Ji ,GUO Chuan-liang ,FAN Shu-yue . 上海交通大学学报（医学版） . 2019,第001期

5. 低氧微环境下胶质瘤干细胞表型的改变与RNA结合蛋白相关 [J] . 李珊珊 ,胡佩珊 ,韩为 . 基础医学与临床 . 2018,第006期

6. 基于多功能纳米载体的小分子药物和小干扰RNA的共释放体系用于促进干细胞的软骨分化并抑制肥大性分化 [C] . 徐建斌 ,黎锦明 ,林思恩 . 2017中国生物材料大会 . 2017

7. RNA结合蛋白DAZAP1促进肝细胞癌进展并通过与SLC7A11 mRNA结合抑制铁死亡的研究 [A] . 王琪 . 2021

1. microRNA-200簇微小RNA在促进骨髓间充质干细胞向成骨细胞分化的应用 [P] . 中国专利： CN111850107A . 2020-10-30

2. 一种miRNA高表达在促进脐带间充质干细胞体外增殖和成骨分化方面的应用 [P] . 中国专利： CN111690686B . 2021.01.15

3. EGL-1, a new protein required for programmed cell death in C. elegans that interacts with the BCL-2-like protein CED-9 [P] . 外国专利： US6596495B1 . 2003-07-22

机译：EGL-1，秀丽隐杆线虫中程序性细胞死亡所需的一种新蛋白质，它与BCL-2-like蛋白CED-9相互作用

4. GENE CODING FOR D40, CASC5 OR SPLICING ISOFORM PROTEIN OF CANCER-TESTIS ANTIGEN PROTEIN, METHOD FOR INDUCING PREVENTION OF PROLIFERATION/MITOSIS OF CANCER CELL AND DEATH OF CELL BY BRINGING THE GENE PRODUCT AS TARGET AND METHOD FOR SCREEING MATERIAL FOR INDUCING PREVENTION OF THE PROLIFERATION/MITOSIS AND DEATH OF THE CELL [P] . 外国专利： JP2007112724A . 2007-05-10

机译：用于D40，CASC5或剪接癌变抗原蛋白的蛋白形式蛋白的基因编码，通过携带基因产物作为靶标来诱导癌细胞增殖/减数分裂和细胞死亡的方法以及寻找用于预防的材料/细胞减数分裂和死亡

5. A novel C. elegans p21-activated kinase (PAK) gene and associated loss-of-function phenotypes that facilitate screening for small molecule modulators of PAK activity in the nematode, Caenorhabditis elegans [P] . 外国专利： AU2005200621B2 . 2010-08-12

机译：新型秀丽隐杆线虫p21激活激酶（PAK）基因和相关的功能丧失表型，有助于筛选线虫秀丽隐杆线虫中PAK活性的小分子调节剂

相关主题

C. elegans RNA-binding proteins PUF-8 and MEX-3 function redundantly to promote germline stem cell mitosis

摘要

著录项

相似文献

相关主题

期刊订阅