Green Fluorescent Fusion Proteins: Powerful Tools for Monitoring Protein Expression in Live Zebrafish Embryos

Kevin G. Peters; Prema S. Rao; Bridgit S. Bell; L.Allen Kindman

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Green Fluorescent Fusion Proteins: Powerful Tools for Monitoring Protein Expression in Live Zebrafish Embryos

机译：绿色荧光融合蛋白：监测活斑马鱼胚胎中蛋白质表达的强大工具

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Therecentdevelopmentoftransgenictechnologyinzebrafishhasopenedanexcitingnewavenueinwhichtoexplorevertebratedevelopment.However,asinotherspecies,theinabilitytoeasilyidentifylivetransgenicfishseverelylimitsthepotentialofthispromisingtechnology.Todeterminewhethertherecentlydescribedgreenfluorescentprotein(GFP)mightprovideaconvenientlivestainingmethodinzebrafish,weconstructedaglutathioneS-transferase/GFPfusionprotein(GST-GFP).GST-GFPcRNA,wheninjectedintoindividualblastomeresofearlyzebrafishembryos,resultedintherapiddevelopment(3hr)ofeasilydetectablegreenfluorescencewhichpersistedforupto4days.GFPfluorescencewasrestrictedtoprogenyoftheinjectedcellandappearedtohavenoadverseeffectsonembryonicdevelopmentdespitewidespreadexpression.OurfindingsdemonstratethatGFPfusionproteinswillprovideasimpleyetpowerfulmeansofmonitoringproductionofheterologousproteinsinlivezebrafish./p/div

机译：Therecentdevelopmentoftransgenictechnologyinzebrafishhasopenedanexcitingnewavenueinwhichtoexplorevertebratedevelopment.However，asinotherspecies，theinabilitytoeasilyidentifylivetransgenicfishseverelylimitsthepotentialofthispromisingtechnology.Todeterminewhethertherecentlydescribedgreenfluorescentprotein（GFP）mightprovideaconvenientlivestainingmethodinzebrafish，weconstructedaglutathioneS转移/ GFPfusionprotein（GST-GFP）.GST-GFPcRNA，wheninjectedintoindividualblastomeresofearlyzebrafishembryos，resultedintherapiddevelopment（3小时）ofeasilydetectablegreenfluorescencewhichpersistedforupto4days.GFPfluorescencewasrestrictedtoprogenyoftheinjectedcellandappearedtohavenoadverseeffectsonembryonicdevelopmentdespitewidespreadexpression.OurfindingsdemonstratethatGFPfusionproteinswillprovideasimpleyetpowerfulmeansofmonitoringproductionofheterologousproteinsinlivezebrafish。

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《Developmental biology》 |1995年第1期|共页
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Kevin G. Peters; Prema S. Rao; Bridgit S. Bell; L.Allen Kindman;
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1. GREEN FLUORESCENT FUSION PROTEINS - POWERFUL TOOLS FOR MONITORING PROTEIN EXPRESSION IN LIVE ZEBRAFISH EMBRYOS [J] . Peters KG., Bell BS., Kindman LA., Developmental biology . 1995,第1期

机译：绿色荧光融合蛋白-监测斑马鱼活体蛋白表达的强大工具
2. Faithful expression of green fluorescent protein (GFP) in transgenic zebrafish embryos under control of zebrafish gene promoters. [J] . Ju B, Xu Y, He J, Developmental genetics . 1999,第2期

机译：在斑马鱼基因启动子的控制下，绿色荧光蛋白（GFP）在转基因斑马鱼胚胎中的忠实表达。
3. THE AEQUOREA VICTORIA GREEN FLUORESCENT PROTEIN CAN BE USED AS A REPORTER IN LIVE ZEBRAFISH EMBRYOS [J] . Amsterdam A., Hopkins N., Lin S. Developmental biology . 1995,第1期

机译：AEQUOREA VICTORIA绿色荧光蛋白可以用作活斑马鱼胚胎的报告者
4. Expression of Multicolor Fluorescent Fusion Proteins in Zebrafish Cell Cultures: A Versatile Tool in Cell Biology [C] . C. K. D. Breek, F. van Iren, S. E. Wijting, Conference on Methods and Applications of Fluorescence: Spectroscopy, Imaging, and Probes . 2002

机译：斑马鱼细胞培养物中多色荧光融合蛋白的表达：细胞生物学的多功能工具
5. Green fluorescent protein in Saccharomyces cerevisiae: Real-time studies of the GAL1 promoter, fusion protein expression and localization/secretion. [D] . Li, Jincai. 2001

机译：酿酒酵母中的绿色荧光蛋白：GAL1启动子，融合蛋白表达和定位/分泌的实时研究。
6. Gαi3 binding to calnuc on Golgi membranes in living cells monitored by fluorescence resonance energy transfer of green fluorescent protein fusion proteins [O] . Thomas S. Weiss, Chester E. Chamberlain, Tetsuro Takeda, 2001

机译：Gαi3与活细胞高尔基体膜上的钙蛋白结合通过绿色荧光共振能量转移进行监测荧光蛋白融合蛋白
7. Green Fluorescent Fusion Proteins: Powerful Tools for Monitoring Protein Expression in Live Zebrafish Embryos [O] . Peters Kevin G., Rao Prema S., Bell Bridgit S., 1995

机译：绿色荧光融合蛋白：监测活斑马鱼胚胎中蛋白质表达的强大工具

Green Fluorescent Fusion Proteins: Powerful Tools for Monitoring Protein Expression in Live Zebrafish Embryos

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