OPA1 functionally interacts with MIC60 but is dispensable for crista junction formation

摘要

Remodelingofcristajunctions(CJs)isobservedinnumeroushumandisordersandduringapoptosis.ThefunctionalinterplayofOPA1andMIC60,twokeyplayersinthiscontext,isunclear.WeshowthatOPA1modulatescristaemorphologybutisdispensableforCJformation.MIC60isstronglyenrichedatCJs,whereasOPA1isdistributedevenlyacrosstheinnermembrane.MIC60levelsareincreasedinOPA1?/?cellswhichshowincreasedcellularresistancetoapoptosisinduction.EndogenousOPA1andMIC60showaphysicalinteraction.Overall,wesuggestthattheregulationofCJremodelingduringapoptosisismediatedviaaninterplaybetweenOPA1andMIC60...