Regulation of chaperone gene expression by heat shock transcription factor in Saccharomyces cerevisiae: Importance in normal cell growth, stress resistance, and longevity

摘要

Heatshocktranscriptionfactor(HSF),akeyregulatorintheexpressionofheatshockprotein(HSP)chaperones,isinvolvedinthemaintenanceofproteinhomeostasis.However,theimpactofHSF‐mediatedtranscriptionofeachHSPgeneonthisprocessisnotfullyunderstood.WeshowthatSaccharomycescerevisiaecellscontainingmutationsintheHSF‐bindingsequencesofchromosomalHSP90promotersexhibitvariousphenotypes,includingslowgrowth,proteotoxicstresssensitivity,andreducedchronologicallifespan.SimilarphenotypeswereobservedwhenHSF‐bindingsequencesinfivemitochondrialHSPpromotersweremutated.Therefore,HSF‐regulatedchangesinexpressionofthesechaperonegenesarenecessarytomaintaincellviabilityundervariousgrowthconditions...