Complementary addressed modification of nucleic acids with the alkylating derivatives of oligothymidylate ethyl phosphotriesters Effect of the phosphotriester fragments' configuration

摘要

>The alkylating derivatives of four individual diastereomers of the oligonucleotide [dTp(Et)]₃dTpU and two individual diastereomers of oligonucleotide [dTp(Et)dTp]₄ have been synthesized. The reagents with the phosphorus atoms in the enantiomeric p″ configuration are shown to be more efficient in reacting with poly(dA) and with nucleic acids in Krebs-2 ascites carcinoma cells compared to those with the phosphorus atoms in the p′ configuration.