On the rapid deprotection of synthetic oligonucleotides and analogs

摘要

The efficiency of ollgodeoxynucleotlde deprotection is greatly enhanced using a combination of: (a) ethanolamlne, and especially a mixture of hydrazine, ethanolamine and methanol, in place of the usual aqueous ammonia; (b) tert-butylphenoxyacetyl amino protecting groups, and (c) oxalyl link between the first nucleotlde and the polymeric support. The extent of base modification, particularly of C, is shown to be extremely low, and the quality of deprotected oligonucleotides is as high as In the case of ammonia deprotection. This method Is also shown to be applicable to the preparation of phosphorothloate and methylphosphonate oligodeoxynucleotldes and oligoribonucleotldes.