Construction of recombinant plasmid carrying the λ DNA fragment responsible for prophage integration

摘要

The recombinant DNA molecules were constructed from plasmid RSF2124 and the EcoRI fragment of λ DNA containing the genes responsible for prophage integration. The presence of these genes in recombinant plasmids was detected genetically. λ int-gene was shown to be expressed in either orientation of insertion in the plasmid. We found that recombinant plasmid was able to integrate into chromosome of λ lysogens. The integration of plasmid into host chromosome was demonstrated by contransduction of chromosome and plasmid markers using generalized transducer P1 and by specialized transduction with λ phages.