Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay

摘要

We assayed Zika virus–infected mosquitoes stored at room temperature for 30 days for live virus by using plaque assay and virus RNA by using real-time reverse transcription PCR. Viable virus was detected in samples stored 10 days, and virus RNA was detected in samples held for 30 days.