Bacterial Whole-Cell Biosensor for Glutamine with Applications for Quantifying and Visualizing Glutamine in Plants

Michael J. Tessaro; Sameh S. M. Soliman; Manish N. Raizada

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Bacterial Whole-Cell Biosensor for Glutamine with Applications for Quantifying and Visualizing Glutamine in Plants

机译：谷氨酰胺的细菌全细胞生物传感器及其在植物中谷氨酰胺定量和可视化中的应用

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A whole-cell biosensor for glutamine (GlnLux) was constructed by transforming an Escherichia coli glutamine (Gln) auxotroph with a constitutive lux reporter gene. Measurements of Gln in plant extracts using GlnLux correlated with quantification using high-performance liquid chromatography (Spearman's r = 0.95). GlnLux permitted charge-coupled-device (CCD) imaging of Gln from whole plant organs.

机译：通过用组成型lux报道基因转化大肠杆菌谷氨酰胺（Gln）营养缺陷型，构建了谷氨酰胺全细胞生物传感器（GlnLux）。使用GlnLux对植物提取物中的Gln进行测量与使用高效液相色谱法（Spearman r = 0.95）的定量相关。 GlnLux允许从整个植物器官对Gln进行电荷耦合器件（CCD）成像。

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《Applied Microbiology》 |2012年第2期|共3页
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Michael J. Tessaro; Sameh S. M. Soliman; Manish N. Raizada;
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1. Bacterial Whole-Cell Biosensor for Glutamine with Applications for Quantifying and Visualizing Glutamine in Plants [J] . Michael J. Tessaro, Sameh S. M. Soliman, Manish N. Raizada Applied and Environmental Microbiology . 2012,第2期

机译：谷氨酰胺的细菌全细胞生物传感器及其在植物中谷氨酰胺定量和可视化中的应用
2. Bacterial Whole-Cell Biosensor for Glutamine with Applications for Quantifying and Visualizing Glutamine in Plants [J] . Michael J. Tessaro, Sameh S. M. Soliman, Manish N. Raizada Applied Microbiology . 2012,第2期

机译：谷氨酰胺的细菌全细胞生物传感器及其在植物中谷氨酰胺定量和可视化中的应用
3. Visualizing Glutamine Accumulation in Root Systems Involved in the Legume–Rhizobia Symbiosis by Placement on Agar Embedded with Companion Biosensor Cells [J] . Malinda S. Thilakarathna, Manish N. Raizada Phytobiomes Journal . 2018,第3期

机译：通过放置嵌入伴伴生物传感器细胞的琼脂，可视化豆类根茎共生中涉及的根系中的谷氨酰胺积累
4. CHARACTERIZING THE EFFECT OF GLUTAMINE SUPPLEMENTATION ON ASPARAGINE AND GLUTAMINE METABOLISM USING ~(13)C METABOLIC FLUX ANALYSIS [C] . Sandra V. Bennun, B. James Kirsch, Amy S. Johnson, Cell culture engineering conference . 2018

机译：〜（13）C代谢通量分析表征补充谷氨酰胺对天冬酰胺和谷氨酰胺代谢的影响
5. Fluorescently Labeled Periplasmic Binding Proteins as Optical Biosensors for Detecting Micromolar Levels of Glucose and Glutamine in Biomedical & Bioprocess Applications [D] . Brown, Sheniqua R. 2019

机译：荧光标记的周质结合蛋白作为光学生物传感器，用于检测生物医学和生物过程应用中的葡萄糖和谷氨酰胺的微摩尔水平
6. Bacterial Whole-Cell Biosensor for Glutamine with Applications for Quantifying and Visualizing Glutamine in Plants [O] . Michael J. Tessaro, Sameh S. M. Soliman, Manish N. Raizada 2012

机译：谷氨酰胺的细菌全细胞生物传感器及其在植物中谷氨酰胺定量和可视化中的应用
7. Bacterial Whole-Cell Biosensor for Glutamine with Applications for Quantifying and Visualizing Glutamine in Plants [O] . Tessaro, Michael J., Soliman, Sameh S. M., Raizada, Manish N. 2011

机译：谷氨酰胺的细菌全细胞生物传感器及其在植物中谷氨酰胺定量和可视化中的应用

Bacterial Whole-Cell Biosensor for Glutamine with Applications for Quantifying and Visualizing Glutamine in Plants

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