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Examination of Market Foods for Coliform Organisms

机译:大肠菌有机食品的检验

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Food specimens (490) in nine categories were examined for total aerobic plate count and numbers and types of coliform organisms, including the enteropathogenic Escherichia coli (EEC). The total counts were compared with various suggested standards, and a limit of 100,000/g appeared to be a realistic goal, except for certain food types with a high level of natural flora. Plate counts in VRB were compared to counts obtained by isolation by enrichment in LST Broth, and the latter method produced a higher percentage of isolations. The presence of E. coli was determined by use of EC Medium incubated at 44.5 ± 0.1 C. Only 40.4% of the positive EC tubes, however, contained E. coli. It appeared that a limit of 10 coliform organisms per g as a suggested standard could be met with several types of foods. Isolation of EEC was obtained only three times, or in 0.6% of the specimens.
机译:对九个类别的食物样本(490)进行了总需氧板数以及大肠菌群(包括肠致病性大肠杆菌(EEC))的数量和类型的检查。将总计数与各种建议的标准进行了比较,限制为100,000 / g似乎是一个现实的目标,除了某些天然菌群较高的食物类型。将VRB中的平板计数与通过LST肉汤富集分离获得的计数进行比较,后一种方法产生的分离百分比更高。通过使用在44.5±0.1 C下孵育的EC培养基来确定大肠杆菌的存在。但是,只有40.4%的阳性EC管含有大肠杆菌。似乎几种食品都可以满足建议的标准,即每克限制10种大肠菌。仅3次获得了EEC的分离,或占标本的0.6%。

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