Newly Discovered Penicillin Acylase Activity of Aculeacin A Acylase from Actinoplanes utahensis

Jesús Torres-Bacete; Daniel Hormigo; Maribel Stuart; Miguel Arroyo; Pedro Torres; María P. Castillón; Carmen Acebal; José L. García; Isabel de la Mata

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Newly Discovered Penicillin Acylase Activity of Aculeacin A Acylase from Actinoplanes utahensis

机译：犹他州猕猴桃的Aculeacin A酰基转移酶新发现的青霉素酰基转移酶活性

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Aculeacin A acylase from Actinoplanes utahensis produced by Streptomyces lividans revealed acylase activities that are able to hydrolyze penicillin V and several natural aliphatic penicillins. Penicillin K was the best substrate, showing a catalytic efficiency of 34.79 mM?1 s?1. Furthermore, aculeacin A acylase was highly thermostable, with a midpoint transition temperature of 81.5°C.

机译：链霉菌青霉菌产生的来自犹他猕猴桃的放线菌素A酰基转移酶显示出能够水解青霉素V和几种天然脂肪族青霉素的酰基转移酶活性。青霉素K是最好的底物，显示出34.79 mM？1 s？1的催化效率。此外，阿古霉素A酰基转移酶高度稳定，中点转变温度为81.5°C。

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《Applied and Environmental Microbiology》 |2007年第16期|共4页
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Jesús Torres-Bacete; Daniel Hormigo; Maribel Stuart; Miguel Arroyo; Pedro Torres; María P. Castillón; Carmen Acebal; José L. García; Isabel de la Mata;
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1. Immobilized aculeacin A acylase from Actinoplanes utahensis: Characterization of a novel biocatalyst [J] . Hormigo D, de la Mata I, Acebal C, Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies . 2010,第12期

机译：固定化的犹他放线菌中的aacleacin A酰基转移酶：新型生物催化剂的表征。
2. A probable aculeacin A acylase from the Ralstonia solanacearum GMI1000 is N-acyl-homoserine lactone acylase with quorum-quenching activity [J] . Chin-Nung Chen, Chii-Jaan Chen, Chen-Ting Liao, BMC Microbiology . 2009,第1期

机译：来自Ralstonia solanacearum GMI1000的可能的aculeacin A酰基转移酶是具有群体猝灭活性的N-酰基-高丝氨酸内酯酰基转移酶
3. Chromogenic analogues of penicillin dihydroF and penicillin K for the continuous spectrophotometric determination of aliphatic penicillin acylase activity [J] . Miguel Arroyo, Raquel Tores-Guzman, Jesus Torres-Bacete, Biotechnology Letters . 2002,第13期

机译：青霉素二氢F和青霉素K的生色类似物，用于连续光度法测定脂肪族青霉素酰基转移酶的活性
4. A penicillin acylase from Streptomyces mobaraensis with a broad specialty on acyl moiety is more similar to non-penicillin acylase in molecular level [C] . China-Japan-Korea Joint Symposium on Enzyme Engineering . 2004

机译：来自茂链链霉菌的青霉素酰基转移酶在酰基部分上具有广泛的专长，在分子水平上与非青霉素酰基转移酶更相似
5. Optimization of recombination methods and expanding the utility of penicillin G acylase. [D] . Loo, Bernard Liat Wen. 2007

机译：优化重组方法并扩大青霉素G酰基转移酶的用途。
6. Newly Discovered Penicillin Acylase Activity of Aculeacin A Acylase from Actinoplanes utahensis [O] . Jesús Torres-Bacete, Daniel Hormigo, Maribel Stuart, 2007

机译：犹他州猕猴桃的Aculeacin A酰基转移酶新发现的青霉素酰基转移酶活性
7. Newly Discovered Penicillin Acylase Activity of Aculeacin A Acylase from Actinoplanes utahensis [O] . García, José Luis, Hormigo, Daniel, Stuart, Maribel, 2007

机译：犹他州猕猴桃的Aculeacin A酰基转移酶新发现的青霉素酰基转移酶活性

Newly Discovered Penicillin Acylase Activity of Aculeacin A Acylase from Actinoplanes utahensis

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