METHOD OF PRODUCING 6-AMINOPENICILLANIC ACID USING THE ACULEACIN A ACYLASE ENZYME OF ACTINOPLANES UTAHENSIS, WHICH IS PURIFIED FROM RECOMBINANT BACTERIA STREPTOMYCES LIVIDANS CECT 3377

摘要

The invention relates to a method of producing 6-aminopenicillanic acid using the aculeacin A acylase enzyme of Actinoplanes utahensis, which is purified from recombinant bacteria Streptomyces lividans CECT 3377. More specifically, the invention relates to a method of producing 6-aminopenicillanic acid, comprising the hydrolysis of penicillins V, K, F or dihydroF, using the aculeacin A acylase enzyme of bacteria Actinoplanes utahensis which is purified from broth cultures of cells of recombinant bacteria Streptomyces lividans CECT 3367 which carry the aac gene that encodes said enzyme.