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首页> 外文期刊>Clinical Chemistry: Journal of the American Association for Clinical Chemists >Generation and characterization of monoclonal antibodies to human type-5 tartrate-resistant acid phosphatase: development of a specific immunoassay of the isoenzyme in serum.
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Generation and characterization of monoclonal antibodies to human type-5 tartrate-resistant acid phosphatase: development of a specific immunoassay of the isoenzyme in serum.

机译:抗5型酒石酸人类酸性磷酸酶单克隆抗体的产生和表征:开发血清中同工酶的特异性免疫测定方法。

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摘要

We have characterized four monoclonal antibodies (mAbs) to the purple ("tartrate-resistant," band 5) acid phosphatase of the human osteoclast (TRAP) and used these to develop a specific serum immunoassay. All four mAbs are of high affinity (Ka = 1-5 x 10(8) L/mol) with a very fast Kassoc (0.2-2.0 x 10(5) L mol-1 s-1) and a moderate Kdissoc (1-3 x 10(-3) s). Two of the mAbs were selected to develop a time-resolved fluorescence immunoassay to measure serum concentrations of TRAP. The mean serum immunoreactive TRAP in a group of healthy premenopausal women and men was 3.7 +/- 1.8 micrograms/L (mean +/- SD) and 3.5 +/- 1.6 micrograms/L, respectively. Significantly higher concentrations of TRAP were found in postmenopausal women (6.3 +/- 2.3 micrograms/L) and in eight patients with Gaucher disease (19.3 +/- 4.7 micrograms/L). Further studies are required to investigate the value of serum TRAP as a marker of bone resorption.
机译:我们已针对人类破骨细胞(TRAP)的紫色(“抗酒石酸”带5)酸性磷酸酶鉴定了四种单克隆抗体(mAb),并使用它们来开发特异性的血清免疫测定法。所有四个mAb具有很高的亲和力(Ka = 1-5 x 10(8)L / mol),具有非常快的Kassoc(0.2-2.0 x 10(5)L mol-1 s-1)和中等的Kdissoc(1 -3 x 10(-3)s)。选择两个mAb进行时间分辨荧光免疫测定,以测量TRAP的血清浓度。一组健康的绝经前男女的平均血清免疫反应性TRAP分别为3.7 +/- 1.8微克/升(平均+/- SD)和3.5 +/- 1.6微克/升。绝经后妇女(6.3 +/- 2.3微克/升)和八名高雪病患者(19.3 +/- 4.7微克/升)发现TRAP的浓度显着升高。需要进一步的研究来研究血清TRAP作为骨吸收标志物的价值。

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