It is now well established that solid tumour growth depends on angiogenesis. However, less is known about the generation of new vessels in haematological malignancies and, in particular, in preleukaemic-myelodysplastic syndromes (MDS). In this study, bone marrow microvessel density (MVD) was assessed by immunohistochemistry and compared in trephine biopsies from 14 controls, five infectious disease (ID), 82 MDS, 15 acute myeloid leukaemia (AML) and 14 myeloproliferative disorder (MPD) patients. Statistical analysis (P P = 0.008). To further investigate angiogenesis machinery, the expression of vascular endothelial growth factor (VEGF) was evaluated by means of immunohistochemistry in control, MDS, AML and MPD biopsies. Even though VEGF mRNA expression was reported in the past in AML cell cultures and cell lines, in our samples VEGF expression was found to be particularly strong in most of the megakaryocytes but significantly less prominent in other cell populations including blasts. Since our findings suggest a correlation between angiogenesis and progression to leukaemia, additional work is now warranted to determine what regulates the generation of new vessels in MDS and leukaemia.
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机译：现在已经确定，实体瘤的生长取决于血管生成。然而，对于血液恶性肿瘤，特别是白血病前骨髓增生异常综合症（MDS）中新血管生成的了解还很少。在这项研究中，通过免疫组织化学评估了骨髓微血管密度（MVD），并在14位对照，5位传染病（ID），82位MDS，15位急性髓性白血病（AML）和14位骨髓增生性疾病（MPD）患者的环烷活检中进行了比较。统计分析（P P = 0.008）。为了进一步研究血管生成机制，通过免疫组织化学方法在对照，MDS，AML和MPD活检中评估了血管内皮生长因子（VEGF）的表达。尽管过去曾在AML细胞培养和细胞系中报道过VEGF mRNA的表达，但在我们的样本中，发现在大多数巨核细胞中VEGF的表达特别强，而在其他细胞群（包括胚细胞）中，VEGF的表达却明显不足。由于我们的发现表明血管生成与白血病的发展之间存在相关性，因此现在有必要开展更多工作来确定什么调节MDS和白血病中新血管的生成。
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