Assessment of Antioxidant Activity of Spray Dried Extracts ofPsidium guajavaLeaves by DPPH and Chemiluminescence Inhibition in Human Neutrophils

摘要

This work evaluated the physicochemical properties and antioxidant activity of spray dried extracts (SDE) fromPsidium guajavaL. leaves. Different drying carriers, namely, maltodextrin, colloidal silicon dioxide, Arabic gum, andβ-cyclodextrin at concentrations of 40 and 80% relative to solids content, were added to drying composition. SDE were characterized through determination of the total phenolic, tannins, and flavonoid content. Antioxidant potential of the SDE was assessed by two assays: cellular test that measures the luminol-enhanced chemiluminescence (LumCL) produced by neutrophils stimulated with phorbol myristate acetate (PMA) and the DPPH radical scavenging (DPPH* method). In both assays the antioxidant activity of the SDE occurred in a concentration-dependent manner and showed no toxicity to the cells. Using the CLlummethod, the IC50ranged from 5.42 to 6.50 µg/mL. The IC50of the SDE ranged from 7.96 to 8.11 µg/mL using the DPPH•method.Psidium guajavaSDE presented significant antioxidant activity; thus they show high potential as an active phytopharmaceutical ingredient. Our findings in human neutrophils are pharmacologically relevant since they indicate thatP. guajavaSDE is a potential antioxidant and anti-inflammatory agent in human cells.